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Synthetic multi-focus optical-resolution photoacoustic microscope for large volumetric imaging

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Document pages: 7 pages

Abstract: Photoacoustic microscopy is becoming an important tool for the biomedicalresearch. It has been widely used in biological researches, such as structuralimaging of vasculature, brain structural and functional imaging, and tumordetection. The conventional optical-resolution photoacoustic microscopy (OR-PAM) employs focused gaussian beam to achieve high lateral resolution by amicroscope objective with high numerical apertures. Since the focused gaussianbeam only has narrow depth range in focus, little detail in depth direction canbe revealed. Here, we developed a synthetic multi-focus optical-resolutionphotoacoustic microscope using multi-scale weighted gradient-based fusion.Based on the saliency of the image structure, a gradient-based multi-focusimage fusion method is used, and a multi-scale method is used to determine thegradient weights. We pay special attention to a dual-scale scheme, whicheffectively solves the fusion problem caused by anisotropic blur andregistration error. First, the structure-based large-scale focus measurementmethod is used to reduce the effect of anisotropic blur and registration erroron the detection of the focus area, and then the gradient weights near the edgewave are used by applying the small-scale focus measure. Simulation wasperformed to test the performance of our method, different focused images wereused to verify the feasibility of the method. Performance of our method wasanalyzed by calculating Entropy, Mean Square Error (MSE) and Edge strength. Theresult of simulation shown that this method can extend the depth of field ofPAM two times without the sacrifice of lateral resolution. And the in vivoimaging of the zebra fish further demonstrates the feasibility of our method.

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