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Computed extended depth of field optical-resolution photoacoustic microscope

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Document pages: 7 pages

Abstract: Photoacoustic microscopy with large depth of focus is significant to thebiomedical research. The conventional optical-resolution photoacousticmicroscope (OR-PAM) suffers from limited depth of field (DoF) since theemployed focused Gaussian beam only has a narrow depth range in focus, littledetails in depth direction can be revealed. Here, we developed a computedextended depth of field method for photoacoustic microscope by using wavelettransform image fusion rules. Wavelet transform is performed on the maxamplitude projection (MAP) images acquired at different axial positions byOR-PAM to separate the low and high frequencies, respectively. The fused lowfrequency coefficients is taking the average of the low-frequency coefficientsof the low-frequency part of the images. And maximum selection rule is used inhigh frequency coefficients. Wavelet coefficient of the MAP images are comparedand select the maximum value coefficient is taken as fused high-frequencycoefficients. And finally the wavelet inverse transform is performed to achievelarge DoF. Simulation was performed to demonstrate that this method can extendthe depth of field of PAM two times without the sacrifice of lateralresolution. And the in vivo imaging of the mouse cerebral vasculature withintact skull further demonstrates the feasibility of our method.

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