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Quantitative phase-contrast imaging a bridge between qualitative phase-contrast and phase retrieval algorithms

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Document pages: 4 pages

Abstract: In the last five decades, iterative phase retrieval methods draw large amountof interest across the research community as a non-interferometric approach torecover quantitative phase distributions from one (or more) intensitymeasurement. However, in cases where a unique solution does exist, thesemethods often require oversampling and high computational resources, whichlimits the use of this approach in important applications. On the other hand,phase contrast methods are based on a single camera exposure but provides onlya qualitative description of the phase, thus are not useful for applications inwhich the quantitative phase description is needed. In this study we adopt acombined approach of the two above-mentioned methods to overcome theirrespective drawbacks. We show that a modified phase retrieval algorithm easilyconverges to the correct solution by initializing the algorithm with aphase-induced intensity measurement, namely with a phase contrast image of theexamined object. Accurate quantitative phase measurements for both binary andcontinuously varying phase objects are demonstrated to support the suggestedsystem as a single-shot quantitative phase contrast microscope.

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