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Inline holographic microscopy through fiber imaging bundles

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Document pages: 7 pages

Abstract: Fiber imaging bundles are widely used as thin, passive image conduits forminiaturised and endoscopic microscopy, particularly for confocal fluorescenceimaging. Holographic microscopy through fiber bundles is more challenging;phase conjugation approaches are complex and require extensive calibration.This articles describes how simple inline holographic microscopy can beperformed through an imaging bundle using a partially coherent illuminationsource from a multimode fiber. The sample is imaged in transmission, with theintensity hologram sampled by the bundle and transmitted to a remote camera.The hologram can then be numerically refocused for volumetric imaging,achieving a resolution of approximately 6 um over a depth range of 1 mm. Thescheme does not require any complex prior calibration and hence is insensitiveto bending.

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