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Green synthesis of silver nanoparticles: structural characteristics and therapeutic effects on Helicobacter pylori gastritis in vivo and in vitro

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Document pages: 11 pages

Abstract: This study evaluated the anti Helicobacter pylori (H.pylori) efficacy of silver nanoparticles (Ag NP) in vivo and in vitro. Silver nanoparticles were prepared through a cost-effective green chemical route, in which Peganum harmala seed extract was used as reducing agent and capping agent. Through surface plasmon resonance spectrophotometry, transmission electron microscopy and powder X-ray diffraction spectroscopy, the structural characteristics show that the synthesized Ag nanoparticles are polydisperse in nature, spherical in shape and have 5-40 pores   nano size. Typical Ag NPs suspension (S5), with a size of 15   nm, showed considerable anti Helicobacter pylori activity when tested on 42 local isolates and 2 reference strains in vitro. In the case of in vivo test of Helicobacter pylori induced gastritis, oral administration6 mg kg body weight of S5 for seven days, a complete clearance was recorded in male albino rates. In comparative time-killing kinetics, S5 exhibited dose- and time-dependent anti-H. pylori activity that was almost similar to tetracycline and clarithromycin, less than amoxicillin, but higher than metronidazole. Furthermore, S5 was found to be an equally effective anti-H. pylori agent at low (≤4) and high pH with no drug resistance observed even up to 10 repeated exposures while a significant drug resistance was recorded for most of the standard drugs employed. The present results revealed the potential of the synthesized Ag-NPs as safer bactericidal agents for the treatment of H. pylori induced gastritis.

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