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Identification code of ZNF191 (243-368) and its interaction with DNA

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Document pages: 7 pages

Abstract: ZNF191 (243-368) is the C-terminal region of ZNF191, which contains four putative DNA binding domains of Cys2His2 zinc finger motifs. In this study, the expression vector of ZNF191 (243-368) and glutathione-S-transferase (GST) fusion protein was constructed and transformed into E. coli BL21. The fusion protein gst-znf191 (243-368) was expressed by the vector, and the protein DNA binding reaction was studied by affinity selection strategy based on the binding quality of zinc finger domain. The results showed that ZNF191 (243-368) could selectively bind to the sequence and react with the gene containing AGGG core. However, the recognition mechanism of Cys2His2 zinc finger protein to DNA needs to be further studied.

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