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Determination of serum total antioxidant capacity by potassium permanganate agar method based on diffusion of serum in agar

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Document pages: 6 pages

Abstract: Goals. To establish a new method for the determination of total antioxidants in serum and evaluate the total antioxidant capacity of organisms. Design and method. The linearity and precision of potassium permanganate agar method were evaluated by sodium thiosulfate (Na2S2O3) and serum. The serum diffusion area of 30 patients in intensive care unit (ICU) and 44 healthy subjects was measured by potassium permanganate agar method. Consequence linearity (0.994 in Na2S2O3 linearity test; 0.987 in serum linearity test) and precision (coefficient of variation of high-level serum diffusion area during operation, operation and daytime, and coefficient of variation of low-level serum diffusion area during operation, operation and operation)-day were all less than 10 ) were acceptable using the potassium permanganate agar method. Total antioxidants of serum between the ICU group and the healthy group were different (, two tailed). Conclusions. Total antioxidants in serum can be determined by the potassium permanganate agar method. The total antioxidant capacity of an organism can be evaluated by the amount of total antioxidants in serum.

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