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Synthesis of graphene oxide palladium nanocomposites functionalized with polydopamine for laccase biosensor

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Document pages: 10 pages

Abstract: Graphene based two-dimensional nano materials have attracted more and more attention in the field of biosensors because of their excellent physical and chemical properties. In this work, a reduced graphene oxide (RGO) hybrid (RGO PD) loaded with palladium nanoparticles (PD) was synthesized by a simple method. Using the self polymerization of dopamine, laccase (LAC) was immobilized on RGO PD to produce polydopamine (PDA). PDA lac RGO PD nanocomposites were further modified on the electrode surface to construct a new biosensor platform. The electrochemical biosensor was applied to the detection of catechol and good analytical results were obtained. Under the optimum conditions, the linear range of the sensor is 0.1   µ m to 263   and the sensitivity is 18.4 for the detection of catechol μ M µ mM−1, and the detection limit was as low as 0.03 µM. In addition, the biosensor also showed good repeatability, reproducibility, anti-interference, and stability. Moreover, the novel Lac based biosensor was successfully used in the trace detection of catechol existing in real water environment.

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