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Effects of his tag on the expression, purification and structure of zinc finger protein ZNF191 (243-368)

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Document pages: 6 pages

Abstract: Zinc finger protein is associated with genetic diseases and cancer. In order to obtain enough zinc finger proteins to study their properties, structure and function, many protein expression systems have been used. ZNF191 (243-368) is a zinc finger protein that can be fused with his markers to produce fusion proteins, such as his6-znf191 (243-368) and ZNF191 (243-368) - his8. Purification of his tag protein with Ni NTA resin can overcome the difficulty of ZNF191 (243-368) separation caused by inclusion body formation. The effects of his labeling on the properties and structure of ZNF191 (243-368) were studied by spectroscopy and hydrolase experiment. Our results show that inserting his tag at the N or C end of ZNF191 (243-368) has different effectson the protein. Therefore, an expression system should be considered based on the properties and structure of the protein. Furthermore, the hydrolase activity of ZNF191(243-368)-His8 has provided new insights into the design of biological functional molecules.

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