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γ Radiation damage to zinc finger of transcription factor IIIA

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Document pages: 7 pages

Abstract: Zinc finger motif is a protein element that can specifically recognize and bind DNA. Because they contain multiple cysteine residues, zinc finger motifs have redox properties. Ionizing radiation produces various free radicals in organisms. Therefore, zinc finger motif may be the target of ionizing radiation. Studied γ Effect of radiation on zinc finger motif in zinc finger protein transcription factor IIIA (TFIIIA). TFIIIA is exposed to different concentrations from 60Co sources γ Dose. The dose rates were 0.20   Gy min and 800   Gy h, respectively. The binding ability of zinc finger motifs in TFIIIA was determined by electrophoretic mobility transfer analysis. We found 1000   γ Ray Gy impairs the function of zinc finger motif in tfiii. The sites of radiation-induced damage in the zinc finger were the thiol groups of cysteine residues and zinc (II) ions. The thiol groups were oxidized to form disulfide bonds and the zinc (II) ions were indicated to be reduced to zinc atoms. These results indicate that the zinc finger motif is a target domain for gamma radiation, which may decrease 5S rRNA expression via impairment of the zinc finger motifs in TFIIIA.

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