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Simple synthesis, characterization and cytotoxic activity of europium doped nano hydroxyapatite

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Document pages: 10 pages

Abstract: The purpose of this study is to synthesize europium doped nano hydroxyapatite by simple water precipitation method, and then characterize and impregnate the selected samples with 5-fluorouracil to explore the properties and release ability of the material. Nano hydroxyapatite doped with 3,5,10 and 20   europium by weight. The resulting samples were characterized after drying at 80 ° C and hydrothermal treatment at 120 ° C for 2 hours. The samples were analyzed by transmission electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy and photoluminescence. In addition, the impregnation and release of 5-fluorouracil were evaluated in PBS. The toxic effects of all samples were studied by measuring the activity of human fibroblasts(GF-1) in vitro. The sizes of the crystallites were about 10–70 nm with irregular morphology and present the phase corresponding to the JCPDS card 9–0432 for hydroxyapatite. The results of the toxicity experiments indicated that doped and undoped powders are biocompatible with fibroblasts cells. Hydroxyapatite samples doped with 5 of europium and loaded with 5-fluorouracil release almost 7 mg L of the drug after 60 minutes in PBS and decrease the viability of HeLa cells after 24 hours.

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