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The role of metals in the catalytic reaction of non-metal ion dependent bacterial ribonuclease

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Document pages: 7 pages

Abstract: Extracellular enzymes of intestinal microbiota are key factors affecting physical functional activities because they interact directly with epithelial and immune cells. Several species of Bacillus, such as Bacillus brevis, a common producer of extracellular ribonuclease binase, can reproduce in the intestinal microbiota as colonizers. Without involving metal ions as cofactors, the duplex enzyme depolymerizes RNA by cleaving the 3 , 5 - phosphate diester bond and generates 2 , 3 - cyclic guanosine phosphate in the first stage of the catalytic reaction. When kept in the reaction mixture for more than one hour, this messenger will affect the human intestinal microflora and human body. In this study, we found that the rate of 2 , 3 - cyclic guanosine acid increased in the presence of transition metalsabilized the RNA structure. At the same time, transition metal ions only marginally reduced the amount of 2′,3′-cGMP, blocking binase recognition sites of guanine at N7 of nucleophilic purine bases.

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