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Synthesis, characterization, cytotoxic activity and interaction with CT-DNA and BSA of cationic ruthenium (II) complexes containing DPPM and quinoline carboxylate

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Document pages: 9 pages

Abstract: The complexes cis - [Ru (QUIN) (DPPM) 2] PF6 and cis - [Ru (kynu) (DPPM) 2] PF6 (quinate; kynu = canine urate; DPPM = bis (diphenylphosphine) methane) were prepared and characterized by elemental analysis, electron, FTIR, 1H and 31P NMR. The characterization data are consistent with the CIS arrangement of DPPM ligands and the bidentate coordination of carboxyloxy groups through QUIN and kynu anions. Circular dichroism showed that these complexes could not be embedded in CT-DNA. On the other hand, the binding constant and thermodynamic parameters of bovine serum albumin (BSA) show that the protein spontaneously interacts with van der Waals force through hydrogen bond. A group of human tumor cell lines including HepG2 and MCF-7 were tested for cytotoxicity and MO59J and one normal cell line GM07492A. In general, the new ruthenium(II) complexes displayed a moderate to high cytotoxicity in all the assayed cell lines with IC50 ranging from 10.1 to 36 µM and were more cytotoxic than the precursor cis-[RuCl2(dppm)2]. The cis-[Ru(quin)(dppm)2]PF6 were two to three times more active than the reference metallodrug cisplatin in the MCF-7 and MO59J cell lines.

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