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Biosynthesis of copper and silver nanoparticles by cyanobacteria Staphylococcus and their antibacterial activities

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Document pages: 9 pages

Abstract: The spread of infectious diseases and the increase of microbial drug resistance have forced researchers to synthesize bioactive nanoparticles. In the field of nano biotechnology, the eco-friendly procedure of nano particle synthesis is becoming more and more perfect. In this study, we used the extract of bottelococcus viridis to synthesize copper and silver nanoparticles. Copper and silver nanoparticles were characterized by UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and scanning electron spectroscopy (SEM). FTIR measurements show that all functional groups can control the reduction and stability of nanoparticles. X-ray diffraction pattern displaythat the particles were crystalline in nature with a face-centred cubic (FCC) geometry. SEM micrographs have shown the morphology of biogenically synthesized metal nanoparticles. Furthermore, these biosynthesized nanoparticles were found to be highly toxic against two Gram-negative bacterial strains Pseudomonas aeruginosa (MTCC 441) and Escherichia coli (MTCC 442), two Gram-positive bacterial strains Klebsiella pneumoniae (MTCC 109) and Staphylococcus aureus (MTCC 96), and a fungal strain Fusarium oxysporum (MTCC 2087). The zone of inhibition was measured by the agar well plate method, and furthermore, minimum inhibitory concentration was determined by the broth dilution assay.

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