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Anticancer potential of green nano silver synthesized from Nepalese Begonia extract on human cervical cancer cells (Hela)

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Document pages: 12 pages

Abstract: In this study, silver nanoparticles (silver nanoparticles) were synthesized using the aqueous extract of Nepalese Begonia plants. The prepared AgNPs (nd AGNPS) were characterized by UV-vis spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, transmission electron microscopy, scanning electron microscopy and energy dispersion spectroscopy. The results obtained from various characterizations show that the average size of the synthesized AgNPs is 33   nm and face centered cubic structure. The anticancer effect of Nd AgNPs on human cervical cancer cells (Hela) was studied. Cytotoxicity was evaluated by 3 - (4,5-dimethylthiazole-2-yl) - 2,5-diphenyltetrazole ammonium bromide (MTT), neutral red uptake (NRU) analysis and morphological changes. Frther, the influence of cytotoxic concentrations of ND-AgNPs on oxidative stress markers, reactive oxygen species (ROS) generation, mitochondrial membrane potential (MMP), cell cycle arrest and apoptosis necrosis was studied. The cytotoxic response observed was in a concentration-dependent manner. Furthermore, the results also showed a significant increase in ROS and lipid peroxidation (LPO), along with a decrease in MMP and glutathione (GSH) levels. The cell cycle analysis and apoptosis necrosis assay data exhibited ND-AgNPs-induced SubG1 arrest and apoptotic necrotic cell death. The biosynthesized AgNPs-induced cell death in HeLA cells suggested the anticancer potential of ND-AgNPs. Therefore, they may be used to treat the cervical cancer cells.

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