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Glutaraldehyde polymerized hemoglobin: looking for improved oxygen carrier performance in bleeding model

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Document pages: 11 pages

Abstract: Hemoglobin - (HB -) based oxygen carrier (HBOC) has been explored for the treatment of bleeding for decades. In our previous supplementary experiments, HBOC with low oxidant reactivity in vitro (peroxidase or serum albumin added for this purpose) showed measurable but slight improvement in parameters related to oxidative stress. Here, this HBOC is tested in a bleeding device; Sheep hemoglobin was also tested in this environment for the first time. Based on in vitro data, compared with bovine hemoglobin, sheep hemoglobin has improved the performance of antioxidant and nitrous stress agents. In fact, sheep hemoglobin performed better than bovine hemoglobin in terms of survival, arterial tension, immunology and histology. On the other hand, different from the supplementary model, in the supplementary model, the non heme peroxidases rubrerythrin and Boine serum albumin copolymerized with Hb were shown to improve the performance of HBOC, in the present hemorrhage models rubrerythrin fails dramatically as HBOC ingredient (with a distinct immunological reaction), whereas serum albumin appears not feasible if its source is a different species (i.e., bovine serum albumin fares distinctly worse than rat serum albumin, in HBOC transfusions in rats). An effect of the matrix in which the HBOCs are dissolved (PBS versus gelofusine versus plasma) is noted.

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