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Boswellia dalzieli mediated silver nanoparticles inhibit Kasumi-1 cells of acute myeloid leukemia (AML) by inducing cell cycle arrest

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Document pages: 11 pages

Abstract: Background acute myeloid leukemia (AML) remains a major health problem, especially in children, because effective chemotherapy against the disease has not yet emerged. Boswellia dalzieli is a famous herbal medicine, which is traditionally used to treat and treat many diseases, including degenerative diseases. In this study, silver nanoparticles were synthesized from phytochemicals in the aqueous extract of Sabina dahuriensis stem bark. Silver nanoparticles were characterized by Fourier transform infrared spectroscopy (FTIR), energy filtered scanning electron microscopy (FESEM), X-ray diffraction and dynamic light scattering (DLS). The antioxidant capacity of nanoparticles was evaluated using 2,2-diphenyl-1-pyridine hydrazide (DPPH) assay and antiproliferative agent effect of the nanoparticles on Kasumi-1 leukemia cells was investigated using PrestoBlue assay. Flow cytometry analysis was performed to observe the effect of the nanoparticles on the leukemia cell cycle progression. Results. Our findings revealed that the synthesized silver nanoparticles were formed from electrons of the plant phytochemicals which include aromatic compounds, ethers, and alkynes. FESEM analysis revealed that the sizes of the nanoparticles range from 12 nm to 101 nm; however, DLS analysis estimated a larger average size of the nanoparticles (108.3 nm) because it measured the hydrodynamic radii of the nanoparticles. The zeta potential of the nanoparticles was −16 nm, and the XRD pattern of the nanoparticles has distinct peaks at 38.02°, 42.94°, 64.45°, 77.20°, and 81.47°, which is typical of face-centered cubic (fcc) structure of silver. The Trolox Equivalence Antioxidant Capacity (TEAC) of the nanoparticles was estimated to be 300.91 μM Trolox mg silver nanoparticles. The nanoparticles inhibited Kasumi-1 cell proliferation. The half minimal inhibitory concentrations (IC50s) that inhibited Kasumi-1 cell proliferation are 49.5 μg ml and 13.25 μg ml at 48 and 72 hours, respectively. The nanoparticles induced cell cycle arrest in the Kasumi-1 cells at S (5 increase) and G2 M (3 increase) phases. Conclusion. The nanoparticles synthesized from the stem bark extract of B. dalzielii inhibit the growth of Kasumi-1 leukemia cells by activating cell cycle arrest; thus, they are potential antileukemic agents.

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