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Synthesis and characterization of green Zinc Oxide nanoparticles and its antiproliferative effect on apoptosis induction and MicroRNA regulation in breast cancer cells

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Document pages: 17 pages

Abstract: The change of microRNA expression will affect the viability and behavior of cancer cells, and have an impact on cancer treatment. In this study, the expression of mir-155-5p, mir-203a-3p and mir-223-3p in MCF7 cancer cell line was studied when exposed to ZnO nanoparticles synthesized through the green pathway. The ZnO nanoparticles were characterized by UV-vis spectroscopy, DLS, XRD, FTIR, FE-SEM, EDX, zeta potential and AFM analysis. Prior to miRNA studies, ZnO NPs were used for cell studies, including MTT cytotoxicity tests for MCF7, MDA-MB-231 and HFF cell lines. In addition, apoptosis was detected by morphological analysis, fluorescent dyes, flow cytometry and Caspase-3 and caspase-8 gene expression evaluation. Biological properties such as antioxidants and antimicrobial activity of these novel ZnO-NPs were considered. MTT assays showed that the inhibitory concentration (IC50) of ZnO-NPs after 24 h was 11.16 μg mL, 60.08 μg mL, and 26.3 μg mL on MCF7, MDA-MB-231, and HFF cells, respectively. The qRT-PCR results showed reduced expression of miR-155-5p, miR-203a-3p, and miR-223-3p when the MCF7 cells were treated with the IC50 concentration of ZnO-NPs (11.16 μg mL). The antioxidant activity results showed EC50 values at 57.19 μg mL and 31.5 μg mL in DPPH and ABTS assays, respectively. The antimicrobial activity of ZnO-NPs was determined on Gram-negative and Gram-positive bacterial strains and fungi using MIC and MBC assays. These NPs had a significant effect in reducing the expression of microRNAs in breast cancer cells. Finally, ZnO-NPs exerted antioxidant and antimicrobial activities.

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