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Identification of ABCA1 differentially expressed genes in HaCaT cells induced by PM2.5 and clarification of pathophysiological correlation

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Document pages: 9 pages

Abstract: Objective to study the effects of PM2.5 on proliferation, cell cycle and apoptosis of human keratinocyte line HaCaT and its possible mechanism. method. HaCaT cells were treated with different concentrations of PM2.5 suspension for 24 hours. The cell viability was detected by CCK-8 method. Cell cycle distribution and apoptosis were detected by flow cytometry. Microarray analysis was used to determine microarray gene expression profiles; Data processing includes gene enrichment and pathway analysis. Western blot was used to verify the key pathways and regulatory factors in microarray analysis. Results with the increase of PM2.5 concentration, cell activity decreased and cell cycle was significantly inhibited. In addition, gene expression microarrays were performed assay, we identified 541 upregulated genes and 935 downregulated genes in PM2.5-treated HaCaT cells. Real-time qPCR and western blot confirmed that PM2.5 treatment could induce the expression of ABCA1 while inhibiting that of END1 and CLDN1. Conclusion. Our results showed that PM2.5 could potentially regulate cell apoptosis and cell cycle arrest via ABCA1-, END1-, ID1-, and CLDN1-mediated pathways in human HaCaT cells, which laid a good foundation for follow-up drug intervention and drug development against skin damage caused by PM2.5 exposure.

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