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Synthesis, DNA binding, anticancer evaluation and molecular docking of dihomogeneous and triheterogeneous ruthenium diimide complexes containing 2 - (2-pyridyl) - quinoxaline

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Document pages: 16 pages

Abstract: Here, we report the synthesis and characterization of Bihomogeneous and triheterogeneous ruthenium (II) polypyridine complexes, namely, [Ru (bpy) 2 (2,2 - PQ)] (PF6) 2 (1) and [Ru (bpy) (phen) (2,2 - PQ)] (PF6) 2 (2), where bpy =   2,2 - bipyridine, phenol =   1,10-phenanthroline and 2,2 - PQ =   2 - (2 - pyridyl) - quinoline. The complexes were characterized by elemental analysis, TGA, 1H-NMR, FT-IR, UV Vis, emission spectroscopy and electrochemistry. Their structures were confirmed by single crystal X-ray diffraction analysis. Complexes 1 and 2 crystallized in orthogonal, PBCA and monoclinic, p21 N systems, respectively. The interaction between the two complexes and calf thymus DNA (CT-DNA) was studied by various spectral techniques. The experimental data are confirmedby molecular docking studies, employing two different DNA sequences. Both complexes, 1 and 2, bind with DNA via a minor groove mode of binding. MTT experiments revealed that both complexes induce apoptosis of MCF-7 (breast cancer) cells in low concentrations. Confocal microscopy indicated that 2 localizes in the nucleus and internalizes more efficiently in MCF-7 than in HEK-293.

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