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Identification and characterization of Arabidopsis L-tyrosine aminotransferase (TAT)

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Document pages: 11 pages

Abstract: The aminotransferase gene family of model plant Arabidopsis consists of 44 genes. Among them, 26 enzymes are classified as characteristic enzymes, which means that the enzyme catalyzed reactions are recorded by experimental means. The remaining 18 enzymes have no characteristics and are therefore considered hypothetical. Our laboratory is interested in clarifying the function of the remaining hypothetical transaminases. To this end, we identified and partially characterized an amino transferase (TAT) from Arabidopsis, which is annotated by the site label at5g36160. The full-length cDNA was cloned and the purified recombinant enzyme was characterized by in vitro and in vivo experiments. In vitro analysis showed that the enzyme could convert L-tyrosine to each othernd 4-hydroxyphenylpyruvate, and L-Phenylalanine and phenylpyruvate. In vivo analysis by functional complementation showed that the gene was able to complement an E. coli with a background of aminotransferase mutations that confers auxotrophy for L-Tyrosine and L-Phenylalanine.

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