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Biofield Energy Based Vitamin D3 Stimulates In Vitro Osteoblasts Differentiation in MG-63 Cell Line

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Document pages: 9 pages

Abstract: In most of the people, bone loss and deterioration of bone structure results in bone disorders such as osteoporosis, arthritis, decreased bone mass, rickets, and deformed bones. Insufficient level of vitamin D3 and calcium in the body are the major causes of bone disorders. The present study aimed to explore the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on various bone health parameters such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization. Both the test items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test items received the Consciousness Energy Healing Treatment by Patricia Margaret Rowe and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of both the sample were denoted as the untreated test items (UT). Cell viability using MTT assay showed that cell viability in tested samples was more than 70 with safe and nontoxic profile on MG-63 cell line. The level of ALP was significantly increased by 230.8 (at 50 µg mL), 231.3 (at 50 µg mL), and 253 (at 100 µg mL) in UT-DMEM BT-TI, BT-DMEM UT-TI, and BT-DMEM BT-TI groups, respectively as compared with the untreated test item and DMEM group. Collagen level was significantly increased by 497.7 , 346.2 , 323 , and 331.4 at 0.1, 1, 10, and 50 µg mL, respectively in the UT-DMEM BT-TI group, while 116.3 and 112.8 at 0.1 and 50 µg mL, respectively in the BT-DMEM UT-TI group. In addition, 70.5 and 143.6 increased collagen was reported at 10 and 50 µg mL, respectively in BT-DMEM BT-TI group as compared with the untreated group. Bone mineralization percentage was significantly increased by 241.3 at 1 µg mL in UT-DMEM BT-TI group, while 187.9 and 113.7 at 0.1 and 1 µg mL, respectively in BT-DMEM UT-TI group as compared with the untreated group. In addition, BT-DMEM BT-TI group showed a significant increased bone mineralization by 129.8 at 1 µg mL as compared with the untreated group. Overall, the experimental data suggested that the Biofield Energy Treated vitamin D3 and DMEM would play an important role in the promotion and maintenance of strong and healthy bones, which improve quality of life. Biofield Energy Treatment might also regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of adverse health conditions, comprising cancer and certain autoimmune diseases.

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