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The Amplification and Application of Ribosomal RNA (rDNA) Gene Sequences of Blidingia minima (Chlorophyta, Blidingia)

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Document pages: 14 pages

Abstract: The sequence of the ribosomal RNA gene (rDNA) playsan important role in species identification and phylogenetic analysis. However,the only published full-length sequence of aribosomal gene of green algae is that of Ulva mutabilis. In thisstudy, we amplified the full-length sequence of eachribosomal gene unit of the ribosomal gene of Blidingia minima. Thefull-length sequence of the ribosomal gene in Blidingia minima is 8676bp, including the 1759 bp 18S rDNA, 576 bp internal transcribed spacer (ITS) + 5.8SrDNA, 3282 bp 28S rDNA, and 3059 bpintergenic spacer (IGS) region. We then carried out a series of geneticanalyses based on the ITS and IGS sequences, to verify whether IGS sequences are useful for studying the geneticdiversity of green algae from different locations. We amplified the IGSsequences of Blidingia minima from 10 different locations in the YellowSea. Multiple alignments of the IGS sequences of samples from these 10 different sites revealed varying degrees of base differences, and comparative analysisof the ITS sequences revealed that our amplified species was classified as Blidingiaminima and distinct from other green algae. In conclusion, our full-lengthamplified ribosomal gene provides useful information to enrich the data ongreen algae ribosomal genes and provides an effective molecular markerfor the analysis of the interspecies and intraspecies relationships of Blidingia minima.

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