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Impact of 17β-Estradiol on Natural Water’s Heterotrophic Nitrifying Bacteria

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Document pages: 12 pages

Abstract: In this research, bottom water samples were collected from nature water. After cultivating and selecting, bacteria which could use (NH4)2SO4 as the only nitrogen source had been selected. The bacteria were cultivated in BM cultures with 0, 0.1, 1, 10, 100 ng L 17β-estradiol (E2), and the initial concentration of E2 is the only difference between cultures of each group. BM culture is a kind of bacteria culture with 100 mg L of NH4-N as only nitrogen source. Every group’s N- NH4+, N-  NO3-concentration and OD600 were measured. The result shows that compared with the control group, in which no E2 was added, the growth of heterotrophic nitrifying bacteria had been promoted when the concentration of E2 was in range of 1 - 100 ng L. In addition, heterotrophic nitrifying bacteria’s growing speed has a positive correlation between the E2’s concentration. However, low concentration of E2 (like 0.1 ng L), could inhibit the growth of heterotrophic nitrifying bacteria. Considering the impact of E2 on heterotrophic nitrifying bacteria, it is necessary to intensify the detection of E2 in the future.

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