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The Effect of a Vascularized Network and Hierarchical Topographical Cue Using a Cell-Electrospinning/3D Printing Process on Inducing Myoblast-Alignment and Maturation

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Document pages: 32 pages

Abstract: The human skeletal muscle is composed of intricate anatomical structures, including uniaxially arranged myotubes and widely distributed blood capillaries. In this regard, vascularization is an essential part of the successful development of an engineered skeletal muscle tissue to restore its function and physiological activities. In this paper, we propose a method to obtain an engineered vascularized skeletal muscle structure developed using cell-electrospinning and 3D bioprinting. To elaborate, on the surface of mechanical supporters (polycaprolactone and collagen struts) with a topographical cue, human umbilical vein endothelial cells (HUVECs)-laden alginate bioink was uniaxially electrospun. The electrospun HUVECs showed high cell viability (90 ), homogeneous cell distribution, and efficient rearrangement into vessels. Furthermore, the myoblasts (C2C12 cells), which were seeded on the vascularized structure (HUVECs-laden fibers), were co-cultured to facilitate myoblast regeneration. As a result, the scaffold that included myoblasts and HUVECs represented a high degree of the myosin heavy chain with striated patterns and enhanced myogenic-specific gene expressions (MyoD, troponin T, and myogenin) as compared to the scaffold that included only myoblasts.

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